Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays
- 1 May 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 7 (5), 486-489
- https://doi.org/10.1128/jcm.7.5.486-489.1978
Abstract
Use of an antigen consisting of purified isolated nuclei from a mixture of human cytomegalovirus-infected and uninfected fibroblasts in a 2:1 ratio is a simple and reliable method for eliminating nonspecific fluorescence associated with the presence of Fc-immunoglobulin G receptors in the cytoplasm of infected cells. The specificity obtained with this antigen on 100 normal human sera was 99, 100 and 98% when compared with microneutralization, anticomplement immunofluorescence and conventional indirect fluorescent-antibody assays, respectively. Also, 95% of the antibody titers obtained with the nuclear antigen had a perfect correlation with or were within a 4-fold-dilution difference of the antibody levels obtained by anticomplement immunofluorescence and the conventional indirect fluorescent-antibody test.This publication has 12 references indexed in Scilit:
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