Acetyl‐Coenzyme‐A Carboxylase of Candida lipolytica
- 1 December 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 71 (1), 301-308
- https://doi.org/10.1111/j.1432-1033.1976.tb11116.x
Abstract
The level of acetyl-coenzyme-A carboxylase activity in C. lipolytica undergoes large variations depending upon the C source on which the yeast is grown. Cells grown on n-alkanes or fatty acids exhibit a lower activity level than do cells grown on glucose. Among the n-alkanes and fatty acids tested, n-heptadecane, n-octadecane, oleic acid and linoleic acid reduce the enzyme activity to the lowest levels, which arew 16-18% of the activity level in glucose-grown cells. Immunochemical titrations and Ouchterlony double-diffusion analysis with specific antibody as well as kinetic studies have indicated that the observed decrease in the level of acetyl-CoA carboxylase activity is due to a reduction in the cellular content of the enzyme. Isotopic leucine incorporation studies with the use of the immunoprecipitation technique demonstrated that the relative rate of synthesis of the enzyme in oleic-acid-grown cells is diminished to 12% of that in glucose-grown cells. Evidence was obtained to support the view that the enzyme in this yeast is not degraded at a rate high enough to contribute to the marked decrease in the cellular content of the enzyme. It is concluded that the reduction in acetyl-CoA carboxylase content in fatty-acid-grown cells is due to diminished synthesis of the enzyme.This publication has 17 references indexed in Scilit:
- Acetyl‐Coenzyme‐A Carboxylase of Candida lipolyticaEuropean Journal of Biochemistry, 1976
- Acetyl-Coenzyme-A Carboxylase from Rat Liver. Subunit Structure and Proteolytic ModificationEuropean Journal of Biochemistry, 1975
- Acetyl‐Coenzyme‐A Carboxylase in Cultured HepatocytesEuropean Journal of Biochemistry, 1975
- Reduction of the acetyl coenzyme A carboxylase content of Saccharomyces cerevisiae by exogenous fatty acidsFEBS Letters, 1973
- Regulation of de novo Fatty Acid Biosynthesis in the n-Alkane-utilizing Yeast, Candida 107Journal of General Microbiology, 1973
- Effects of Chain-length of Alkane Substrate on Fatty Acid Composition and Biosynthetic Pathway in Some Candida YeastsAgricultural and Biological Chemistry, 1973
- Fatty acid and hydrocarbon hydroxylation in yeast: Role of cytochrome P-450 in Candida tropicalisBiochemical and Biophysical Research Communications, 1971
- Role of Acetyl Coenzyme A Carboxylase in the Control of Fatty Acid SynthesisPublished by Elsevier ,1971
- An improvement in a procedure for counting tritium and carbon-14 in polyacrylamide gelsAnalytical Biochemistry, 1970
- Regulatory Effects of Nicotinamide on Tryptophan Pyrrolase Synthesis in Rat Liver in vivoEuropean Journal of Biochemistry, 1968