Isolation of oligonucleosomes from active chromatin using HMG17-specific monoclonal antibodies
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (8), 3363-3376
- https://doi.org/10.1093/nar/14.8.3363
Abstract
We report the preparation of HM017-containing oligonucleosomes from chicken embryos and from liver and oviduct of laying hens. Monoclonal antibodies against HM017 were used for their isolation. An unusual size distribution with respect to their repeat number was observed. The oligonucleosomes of repeat number up to N6 were highly enriched for DNA of the vitellogenin II gene (liver) and for DNA of the ovalbumin and lysozyme genes (oviduct).Keywords
This publication has 16 references indexed in Scilit:
- Primary organization of nucleosomesJournal of Molecular Biology, 1985
- Chromatin structure of hsp 70 genes, activated by heat shock: Selective removal of histones from the coding region and their absence from the 5′ regionCell, 1984
- Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research, 1984
- A faithful double stain of proteins in the polyacrylamide gels with Coomassie blue and silverAnalytical Biochemistry, 1982
- One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-gel blue chromatographyJournal of Immunological Methods, 1982
- Function of a tRNA gene promoter depends on nucleosome positionNature, 1982
- Chromatin Structure and Gene Activity: The Role of Nonhistone Chromosomal ProteinCritical Reviews in Biochemistry, 1982
- Isolation of actively transcribed nucleosomes using immobilized HMG 14 and 17 and an analysis of α-globin chromatinCell, 1981
- The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activityCell, 1979
- Nucleosome mono-, di-, tri-, and tetramers from chicken embryo chromatinNucleic Acids Research, 1977