Factors Controlling Packaging of Peptide Hormones into Secretory Granulesa
- 1 April 1987
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 493 (1), 50-61
- https://doi.org/10.1111/j.1749-6632.1987.tb27180.x
Abstract
Endocrine, exocrine, and neuronal cells package only a subset of their secretory products into the electron-dense secretory granules. To investigate the factors controlling selective packaging of proteins into these granules, we utilized the mouse pituitary tumor cell line, AtT-20, which retained the capability to sort adrenocorticotropic hormone (ACTH) into secretory granules in vitro. Packaging of ACTH was blocked by treatment with weak bases, but was unaffected when N-linked glycosylation or sulfation was inhibited. To test whether the targeting information is specified by sorting domains present on peptide hormone sequences, we determined if a protein could be diverted to the dense secretory granules by attachment to a peptide hormone sequence. A plasmid DNA was constructed that encoded a hybrid protein in which a fragment of a viral membrane protein was fused to the carboxy terminus of human growth hormone. AtT-20 cells transfected with the hybrid were found to target it to dense secretory vesicles efficiently. These results support the hypothesis that sorting domains on peptide hormones direct their packaging into dense secretory vesicles.This publication has 25 references indexed in Scilit:
- Secretory protein targeting in a pituitary cell line: differential transport of foreign secretory proteins to distinct secretory pathways.The Journal of cell biology, 1985
- Selective packaging of human growth hormone into synaptic vesicles in a rat neuronal (PC12) cell line.The Journal of cell biology, 1985
- The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer.The Journal of cell biology, 1985
- Sorting and secretion of adrenocorticotropin in a pituitary tumor cell line after perturbation of the level of a secretory granule-specific proteoglycan.The Journal of cell biology, 1984
- Somatostatin discriminates between the intracellular pathways of secretory and membrane proteins.The Journal of cell biology, 1984
- Vesicular stomatitis virus glycoprotein, albumin, and transferrin are transported to the cell surface via the same Golgi vesicles.The Journal of cell biology, 1983
- Chloroquine diverts ACTH from a regulated to a constitutive secretory pathway in AtT-20 cellsNature, 1983
- The secretory pathway in yeastTrends in Biochemical Sciences, 1982
- Rat Pro-Opiomelanocortin Contains SulfateScience, 1982
- Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein.The Journal of cell biology, 1981