SEPARATION OF BILE ACID CONJUGATES BY ION EXCHANGE CHROMATOGRAPHY
- 1 January 1963
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 41 (1), 77-89
- https://doi.org/10.1139/o63-010
Abstract
A simple and reliable method for the separation of bile acid conjugates in quantities ranging from a few milligrams to several grams has been developed utilizing ion exchange resins. The bile acid conjugates were absorbed from an aqueous 25% ethanol solution on a Dowex-1 anion exchange column (acetate form). After the column was washed with aqueous 25% ethanol, the glycine conjugates were eluted with 0.05 N HCl in 25% ethanol and the taurine conjugates with 0.5 N HCl in 25% ethanol. The method permitted a complete resolution of the glycine and taurine conjugates. In addition, there was an effective though not complete separation of the tri- and di-hydroxy cholanic acid derivatives within each conjugate group. Using this method satisfactory fractionations have been performed on gallbladder bile from dog, ox, and man.Keywords
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