The inhibitory effect of human interferon α on the generation of lymphokine-activated killer activity
- 1 July 1989
- journal article
- research article
- Published by Springer Nature in Cancer Immunology, Immunotherapy
- Vol. 30 (4), 205-212
- https://doi.org/10.1007/bf01665006
Abstract
The generation of lymphokine-activated killer (LAK) activity and the proliferative response to human recombinant interleukin-2 (IL-2) were significantly reduced by the presence of human recombinant leukocyte interferon (IFNα) in cultures of human peripheral blood mononuclear cells (PBMC). Mature natural killer (NK) cells can be depleted from PBMC with the toxic lysosomotropic agentl-leucine methyl ester. The generation of cytotoxic cells from lymphocytes depleted in leucine methyl ester was also inhibited by indicating that the IFN-α effect is not limited to mature cytotoxic NK cells. Depletion of adherent cells from PBMC did not affect the suppression of LAK induction by IFN-α. Surface marker analyses of Tac antigen and transferrin receptor (TfR) showed that the presence of IFNα throughout the culture period significantly suppressed the typical increase in IL-2-induced Tac- and TfR-positive cells. In contrast, IFNα treatment before and after IL-2 culture enhanced LAK cytotoxic activity. Therefore, combinations of these biological response modifiers for clinical use should take into account the dual effect of IFNα on key features of the IL-2 response.Keywords
This publication has 49 references indexed in Scilit:
- Combination Immunotherapy for Cancer:Synergistic Antitumor Interactions of Interleukin-2, Alfa Interferon, and Tumor-Infiltrating LymphocytesJNCI Journal of the National Cancer Institute, 1988
- The regulatory effect of adherent cells on lymphokine activated killer cellsCellular Immunology, 1987
- A New Approach to the Adoptive Immunotherapy of Cancer with Tumor-Infiltrating LymphocytesScience, 1986
- Regulation of interferon receptor expression in human blood lymphocytes in vitro and during interferon therapy.JCI Insight, 1986
- The role of transferrin in natural killer cell and IL-2-induced cytotoxic cell functionCellular Immunology, 1985
- Observations on the Systemic Administration of Autologous Lymphokine-Activated Killer Cells and Recombinant Interleukin-2 to Patients with Metastatic CancerNew England Journal of Medicine, 1985
- Functional studies on the precursors of human lymphokine-activated killer cellsCellular Immunology, 1985
- Close link between reduction of c-myc expression by interferon and G0/G1 arrestNature, 1985
- Effect of Recombinant Alpha Interferon on NK and ADCC Function in Lung Cancer Patients: Results from a Phase II TrialJournal of Interferon Research, 1984
- Human leukocyte interferon produced by E. coli is biologically activeNature, 1980