Characteristic Signatures of the lytA Gene Provide a Basis for Rapid and Reliable Diagnosis of Streptococcus pneumoniae Infections
- 1 April 2006
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (4), 1250-6
- https://doi.org/10.1128/jcm.44.4.1250-1256.2006
Abstract
The nucleotide sequences of the lytA gene from 29 pneumococcal isolates of various serotypes and 22 additional streptococci of the mitis group (including two Streptococcus pseudopneumoniae strains) have been compared and found to correspond to 19 typical (927-bp-long) and 20 atypical (921-bp-long) alleles. All the Streptococcus pneumoniae strains harbored typical lytA alleles, whereas nonpneumococcal isolates belonging to the mitis group always carried atypical alleles. A sequence alignment showed that the main difference between typical and atypical lytA alleles resided in 102 nucleotide positions (including the 6 bp absent from atypical alleles). These nucleotides were perfectly conserved in all the typical alleles studied, and the corresponding nucleotides of the atypical alleles were also perfectly conserved. The presence in these signatures of distinctive restriction sites (namely, SnaBI, XmnI, and BsaAI) allowed the development of a simple, reliable, and fast method that combines PCR amplification of the lytA gene, digestion with BsaAI, and separation of the products by agarose gel electrophoresis. This assay allows the rapid and consistent identification of true S. pneumoniae strains and represents an improved diagnostic tool for the study of pneumococcal carriage.Keywords
This publication has 50 references indexed in Scilit:
- Analysis of the Genetic Structure of Nontypeable Pneumococcal Strains Isolated from ConjunctivaJournal of Clinical Microbiology, 2005
- Characterization of LytA-LikeN-Acetylmuramoyl-l-Alanine Amidases from Two NewStreptococcus mitisBacteriophages Provides Insights into the Properties of the Major Pneumococcal AutolysinJournal of Bacteriology, 2004
- Recent trends on the molecular biology of pneumococcal capsules, lytic enzymes, and bacteriophageFEMS Microbiology Reviews, 2004
- Comparison of four polymerase chain reaction assays for specificity in the identification of Streptococcus pneumoniaeDiagnostic Microbiology and Infectious Disease, 2004
- VO1, a Temperate Bacteriophage of the Type 19A Multiresistant Epidemic 8249 Strain ofStreptococcus pneumoniae: Analysis of Variability of Lytic and Putative C5 Methyltransferase GenesMicrobial Drug Resistance, 2003
- Molecular Peculiarities of the lytA Gene Isolated from Clinical Pneumococcal Strains That Are Bile InsolubleJournal of Clinical Microbiology, 2002
- Sensitive and Specific Method for Rapid Identification of Streptococcus pneumoniae Using Real-Time Fluorescence PCRJournal of Clinical Microbiology, 2001
- Optochin Resistance inStreptococcus pneumoniae:Mechanism, Significance, and Clinical ImplicationsThe Journal of Infectious Diseases, 2001
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Molecular basis of the optochin‐sensitive phenotype of pneumococcus: characterization of the genes encoding the F0 complex of the Streptococcus pneumoniaeStreptococcus oralis H+‐ATPasesMolecular Microbiology, 1994