The Collection, Storage and Preparation of Viable Cadaver Marrow for Intravenous Use
Open Access
- 1 February 1959
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 14 (2), 140-147
- https://doi.org/10.1182/blood.v14.2.140.140
Abstract
A procedure for collecting and storing viable cadaver marrow in a form suitable for intravenous use is described. Vertebral bone marrow is removed under sterile conditions within a few hours after death. The marrow cells are screened and suspended after centrifugation in an equal volume of a liquid medium consisting of 30 per cent glycerol and 70 per cent human serum and frozen to -80 C. in plastic bags. Suggestions for thawing, deglycerolizing and administering the cells intravenously are given.Keywords
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