MICRODETERMINATION OF A Δ4–3 KETOSTEROID (PROGESTERONE) OBTAINED FROM SMALL VOLUMES OF SERUM1

Abstract

Absorption curves for a number of steroids were detd., and found to agree with those in the literature. By using selective chemical procedures, one may obtain in one fraction the neutral, non-volatile compounds of high molecular wt., with appropriate chromogenic activity for progesterone limited to the ketonic fraction along with estrogens. The characteristics of progesterone, and estrogen absorption curves are such that the 2 hormones may be measured simultaneously. The test for progesterone is characteristic for [alpha], [beta] unsaturated ketones; by employing suitable chemical procedures, one may be reasonably certain that he is dealing with [DELTA]4-3 ketosteroids. The method does not distinguish between testosterone, corticosterone and related substances, adrostenedione and progesterone. As developed at present, the method is limited to those exptl. conditions in which it is certain or highly probable that he is dealing with progesterone, or one of the other specific, [DELTA]4-3-ketosteroids. The limitations, sensitivity and details for improvement are outlined.