A v-SNARE implicated in intra-Golgi transport.
Open Access
- 1 May 1996
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 133 (3), 507-516
- https://doi.org/10.1083/jcb.133.3.507
Abstract
We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, A Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles.Keywords
This publication has 42 references indexed in Scilit:
- Dissection of a single round of vesicular transport: Sequential intermediates for intercisternal movement in the Golgi stackCell, 1989
- Involvement of GTP-binding “G” proteins in transport through the Golgi stackCell, 1987
- The trans Golgi Network: Sorting at the Exit Site of the Golgi ComplexScience, 1986
- Application of cryoultramicrotomy to immunocytochemistryJournal of Microscopy, 1986
- Isolation of monoclonal antibodies specific for products of avian oncogene myb.Molecular and Cellular Biology, 1984
- Sequential intermediates in the pathway of intercompartmental transport in a cell-free systemCell, 1984
- Reconstitution of the transport of protein between successive compartments of the golgi measured by the coupled incorporation of N-acetylglucosamineCell, 1984
- Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.The Journal of cell biology, 1982
- Purification and characterization of a rat liver Golgi alpha-mannosidase capable of processing asparagine-linked oligosaccharides.Journal of Biological Chemistry, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970