Improved Detection of Bartonella DNA in Mammalian Hosts and Arthropod Vectors by Real-Time PCR Using the NADH Dehydrogenase Gamma Subunit ( nuoG )
- 1 December 2010
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 48 (12), 4630-4633
- https://doi.org/10.1128/jcm.00470-10
Abstract
We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (nuoG) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.Keywords
This publication has 27 references indexed in Scilit:
- Run-Off Replication of Host-Adaptability Genes Is Associated with Gene Transfer Agents in the Genome of Mouse-Infecting Bartonella grahamiiPLoS Genetics, 2009
- Transmission ofBartonella henselaebyIxodes ricinusEmerging Infectious Diseases, 2008
- Bartonella tamiae sp. nov., a Newly Recognized Pathogen Isolated from Three Human Patients from ThailandJournal of Clinical Microbiology, 2008
- False-Negative Results in Nucleic Acid Amplification Tests—Do We Need to Routinely Use Two Genetic Targets in all Assays to Overcome Problems Caused by Sequence Variation?Critical Reviews in Microbiology, 2008
- Acquisition of nonspecific Bartonella strains by the northern grasshopper mouse (Onychomys leucogaster)FEMS Microbiology Ecology, 2007
- Emerging zoonoses and vector-borne infections affecting humans in EuropeEpidemiology and Infection, 2007
- Genome Rearrangements, Deletions, and Amplifications in the Natural Population of Bartonella henselaeJournal of Bacteriology, 2006
- Bartonella Infection in Domestic Cats and Wild FelidsAnnals of the New York Academy of Sciences, 2006
- A Bartonella vinsonii berkhoffii typing scheme based upon 16S–23S ITS and Pap31 sequences from dog, coyote, gray fox, and human isolatesMolecular and Cellular Probes, 2006
- Functional Divergence and Horizontal Transfer of Type IV Secretion SystemsMolecular Biology and Evolution, 2005