The hexokinase activity of animal tumours

Abstract

The hexokinase activity of Jensen rat sarcoma, Walker 256 carcinoma, S 37 mouse carcinoma, Crocker 180 mouse carcinoma was sufficient to account for all the glucose used in the anaerobic glycolysis of tumor slices passing through the hexokinase reaction. The tumor enzyme phosphorylated glucose and mannose readily. For opt. phosphorylation of fructose a higher concn. was required. Galactose was not phosphorylated. The enzyme was inhibited in vitro by methylbis (2-chloroethyl) amine but not by other radiomimetic mitotic poisons which react more slowly.