Studies in tissue metabolism

Abstract

Addition of yeast cozymase and adenylic acid to a cell-free extract of the Crocker 180 tumor increases the glycolytic power to that of the tissue from which the extract was made. In the presence of added cozymase and adenylic acid, glucose, hexosemono-phosphate and hexosediphosphate are converted into lactic acid in the extract as rapidly as glucose by the original tissue. Cozymase and adenylic acid are, however, rapidly destroyed by the extract. Extracts of boiled tumor tissue increase the rate of fermentation by washed dried yeast and therefore contain a cozymase.