Comparison of a Fluorimetric Method and a Competitive Protein Binding Assay Kit for the Determination of Plasma Hydroxycorticosteroids
- 1 January 1975
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 12 (1-6), 160-162
- https://doi.org/10.1177/000456327501200138
Abstract
A new competitive protein binding (C.P.B.) assay kit for the determination of plasma hydroxycorticosteroids which uses a gamma emitting isotope, selenium-75, to label the Cortisol was compared with a fluorimetric method in use in a routine laboratory. The mean plasma corticosteroid level in a group of 54 normal subjects was found to be lower with the C.P.B. kit than with the fluorimetric method. The correlation coefficient between the two methods in 131 specimens from healthy subjects and patients under investigation for pituitary or adrenocortical disorders was + 0.92. The precision of the two methods was similar.Keywords
This publication has 3 references indexed in Scilit:
- A simple competitive protein binding assay for plasma cortisolClinica Chimica Acta; International Journal of Clinical Chemistry, 1974
- Comparison of Results by Four Different Procedures for Determination of 17-HydroxycorticosteroidsClinical Chemistry, 1973
- Some Studies of the Protein-Binding of Steroids and Their Application to the Routine Micro and Ultramicro Measurement of Various Steroids in Body Fluids by Competitive Protein-Binding Radioassay1Journal of Clinical Endocrinology & Metabolism, 1967