Influence of Hemagglutinating Viruses on Tumor Cell Suspensions: II. Newcastle Disease Virus and Ehrlich Carcinoma.

Abstract

The Ehrlich mouse carcinoma was made into single cell suspensions mixed with an equal portion of Newcastle disease allantoic fluid virus, and inoculated either subcut. or intraperit. into mice. Inhibition of tumor growth was noted immediately after mixing at 4[degree], 25[degree] and 37[degree]. The tumor inhibition extended for periods of 5 hrs. at both 4[degree] and 37[degree] but at 25[degree] the mixtures regained their ability to grow after 1-2 hrs. All normal allantoic fluid tumor cell mixtures grew under the same circumstances. These results are comparable to those obtained with sarcoma 180 cell, NDV mixtures. When the virus and cell suspensions were mixed in vivo no tumor inhibition was noted. Since the virus failed to grow in either the solid Ehrlich tumor or the ascitic forms the results are discussed as being analogous to the hemagglutination phenomenon.