HOST SPECIFICITY OF DNA PRODUCED BY Escherichia coli , XI. In vitro MODIFICATION OF PHAGE FD REPLICATIVE FORM
- 1 June 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 63 (2), 556-562
- https://doi.org/10.1073/pnas.63.2.556
Abstract
An enzymatic activity, having the properties expected of a B-specific host-controlled modification enzyme, has been purified from an extract of Escherichia coli strain B. This activity renders the unmodified replicative form of phage fd resistant to B-specific restriction and is only present in strains carrying intact genes for type B modification. In phosphate buffer, the enzyme acts optimally at pH 6 and is dependent upon a single cofactor, S-adenosylmethionine.Keywords
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- EIN FADIGER DNS-PHAGE (FD) UND EIN SPHARISCHER RNS-PHAGE (FR), WIRTSSPEZIFISCH FUR MANNLICHE STAMME VON E. COLI .1. PRAPARATION UND CHEMISCHE EIGENSCHAFTEN VON FD UND FR1963