Functional groups on ‘Z’ DNA recognized by monoclonal antibodies
- 26 March 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 168 (2), 303-306
- https://doi.org/10.1016/0014-5793(84)80267-7
Abstract
Both brominated poly[d(GC)] and poly[d(Gm5C)] form stable left‐handed Z‐DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid‐phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non‐specific antibodies but two types of very specific antibody were also distinguished. The first binds poly[d(Gm5C)] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer.Keywords
This publication has 14 references indexed in Scilit:
- Z-DNA immunoreactivity in fixed metaphase chromosomes of primates.Proceedings of the National Academy of Sciences, 1983
- Isolation of Z-DNA-containing plasmids.Proceedings of the National Academy of Sciences, 1983
- Isolation of Drosophila proteins that bind selectively to left-handed Z-DNA.Proceedings of the National Academy of Sciences, 1982
- Interaction between antibodies to Z-form deoxyribonucleic acid and double-stranded polynucleotidesBiochemistry, 1982
- Specificity of autoimmune monoclonal Fab fragments binding to single-stranded deoxyribonucleic acidBiochemistry, 1982
- A method for the specific Inhibition of poly[d(A-T)] synthesis using the A-T specific quinoxaline antibiotic TANDEMCanadian Journal of Biochemistry, 1982
- Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomesNature, 1981
- Antibodies specific for left-handed Z-DNA.Proceedings of the National Academy of Sciences, 1981
- Monoclonal antibodies showing sequence specificity in their interaction with single-stranded DNAsNucleic Acids Research, 1981
- Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).Proceedings of the National Academy of Sciences, 1981