PARTIAL PURIFICATION OF HUMAN PROTHROMBIN AND PROCONVERTIN: THEIR CHARACTERISTICS AND INTERACTION 1

Abstract

A method for the prepn. of prothrombin and proconvertin from oxalated human plasma is presented. Activation of prothrombin in the presence of Ca, thromboplastin, and accelerin revealed that the yield of thrombin was dependent upon the initial concn. of prothrombin, and that the rate of thrombin formation was dependent upon the initial concn. of proconvertin. Proconvertin could be activated to convertin by Ca and thromboplastin. The lag phase usually observed in thrombin formation was eliminated when prothrombin was activated in the presence of tissue thromboplastin, convertin, and accelerin.