Chromosomes of human hepatoma cell lines

Abstract

The karyotypes of three human hepatoma cell lines Hep G2, Hep 3B and PLC/PRF/5 were investigated by G‐ and C‐banding techniques. In addition to ploidy changes, typical for most carcinoma cell lines, certain markers were found that remained stable throughout passage of these cultures. Chromosome I is involved in multiple translocations, resulting in at least three copies of the chromosome I heterochromatin region in each cell line. Inversion in the 9qh region is also seen. In addition, each of the cell lines consistently contains trisomy of 17q. The rearrangements of chromosome I are most striking in the Hep 3B and PLC/PRF/5 cell lines, which are derived from human hepatocellular carcinomas and contain integrated copies of the hepatitis B viral genome. These two cell lines are characterized by the presence of at least five copies of the I (p13←q21) region that result from multiple deletions and/or translocations; by consistent trisomy and polymorphism of the 9qh region; and by trisomy of chromosome 10 (also involved in rearrangements). The Hep G2 and Hep 38 cell lines behave functionally as highly differentiated liver parenchymal cells and are karyologically distinguishable from PLC/PRF/5 both by the presence of trisomy of 6 (pter←q14) and by the finding that one of the homologues of chromosome 15 is 15q+.