Basic amino acid transport in plasma membrane vesicles of Penicillium chrysogenum
- 1 July 1996
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 178 (14), 3991-3995
- https://doi.org/10.1128/jb.178.14.3991-3995.1996
Abstract
The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart mitochondrial cytochrome c oxidase. In the presence of reduced cytochrome c, the hybrid membranes accumulated the basic amino acids arginine and lysine. Inhibition studies with analogs revealed a narrow substrate specificity. Within the external pH range of 5.5 to 7.5, the transmembrane electrical potential (delta psi) functions as the main driving force for uphill transport of arginine, although a low level of uptake was observed when only a transmembrane pH gradient was present. It is concluded that the basic amino acid permease is a H+ symporter. Quantitative analysis of the steady-state levels of arginine uptake in relation to the proton motive force suggests a H+-arginine symport stoichiometry of one to one. Efflux studies demonstrated that the basic amino acid permease functions in a reversible manner.Keywords
This publication has 21 references indexed in Scilit:
- Structural and Functional Properties of Plasma Membranes from the Filamentous Fungus Penicillium chrysogenumEuropean Journal of Biochemistry, 1994
- Secondary solute transport in bacteriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1993
- Proton-Coupled Sugar and Amino Acid Transporters in PlantsAnnual Review of Plant Physiology and Plant Molecular Biology, 1993
- Transport of neutral amino acids and penicillin formation in Penicillium chrysogenumJournal of General Microbiology, 1990
- Kinetic properties, nutrient-dependent regulation and energy coupling of amino-acid transport systems in Penicillium cyclopiumBiochimica et Biophysica Acta (BBA) - Biomembranes, 1989
- Amino acid transport in eucaryotic microorganismsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1986
- The effect of ‘probe binding’ on the quantitative determination of the proton-motive force in bacteriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1982
- Induction of penicillin biosynthesis by L-glutamate in Penicillium chrysogenumBiochemical and Biophysical Research Communications, 1982
- Amino acid transport into fungi: An essayTransactions of the British Mycological Society, 1976
- Specificity of transinhibition of amino acid transport in neurosporaBiochemical and Biophysical Research Communications, 1971