Recovery of rat submandibular salivary gland function following removal of obstruction: a sialometrical and sialochemical study
- 24 November 2006
- journal article
- Published by Wiley in International Journal of Experimental Pathology
- Vol. 87 (6), 411-423
- https://doi.org/10.1111/j.1365-2613.2006.00500.x
Abstract
Functional recovery of the rat submandibular gland following ligation of the main excretory duct was examined. Rat submandibular glands were ligated for 1, 4 and 8 weeks using a micro-clip with a plastic tube. Micro-clips were removed and glands were allowed to recover for periods of 8, 16 and 24 weeks. Submandibular glands were stimulated with autonomimetic drugs (methacholine and isoprenaline) and salivas were collected from atrophic or de-ligated and contralateral control glands. Glands recovered almost full size (92% of control gland) following 24 weeks of de-ligation. Saliva volume secreted by ligated/de-ligated (RSM) and control (LSM) glands were similar with different doses of agonists. Protein output expressed per gram of tissue wet weight was similar from both ligated/de-ligated and control glands with all doses of agonist. Sodium and chloride levels were higher from de-ligated glands than contralateral control glands. Protein electrophoresis showed similar profiles of salivary proteins in all samples with some minor differences. Acinar cells in de-ligated glands showed a normal morphology, as indicated by light microscopy, whilst granular ductal cells were fewer and contained fewer secretory granules. Sodium potassium ATPase staining of striated ducts in de-ligated glands was similar to that of control glands. It can be concluded that rat submandibular glands can regenerate following severe atrophy and secrete normal amounts of saliva containing broadly a full profile of secretory proteins. In contrast to acinar cells, ductal cells appear not to recover full function.Keywords
This publication has 48 references indexed in Scilit:
- Intraoral duct ligation without inclusion of the parasympathetic nerve supply induces rat submandibular gland atrophyInternational Journal of Experimental Pathology, 2006
- Modern management of salivary calculiBritish Journal of Surgery, 2005
- Origin of acinar cell regeneration after atrophy of the rat parotid induced by duct obstructionInternational Journal of Experimental Pathology, 1998
- Control of SalivationPublished by S. Karger AG ,1998
- Nerve growth factor effects on inhibition by submandibular gland ablation or autonomic denervation of activity-induced parotid growthJournal of the Autonomic Nervous System, 1992
- Quantitative assessment of Na+,K+-ATPase localization by direct and indirect p-nitrophenyl phosphatase methods.Journal of Histochemistry & Cytochemistry, 1987
- Submaxillary gland recovery from obstruction. II. Electron microscopic alterations of acinar cellsJournal of Ultrastructure Research, 1971
- Submaxillary gland recovery from obstruction. I. Overall changes and electron microscopic alterations of granular duct cellsJournal of Ultrastructure Research, 1971
- Quantitative determination of protein in salivaEuropean Journal of Oral Sciences, 1971
- Regeneration of the Salivary Glands in the RabbitJournal of Dental Research, 1966