Effect of vitamin A compounds on the covalent binding of benzo(a)pyrene to nuclear macromolecules.

Abstract

Effect of vitamin A compounds on the covalent binding of benzo (a) pyrene [B (a) P] to subnuclear components was studied using rat liver nuclei in vitro. The retinol-induced inhibition of the covalent binding of B (a) P depended both on the dose and the time of addition. Retinal and retinyl acetate were both potent inhibitors, but retinoic acid was less effective. Retinol induced marked inhibition of B (a) P binding to DNA and protein of the nuclear matrix but had little or no effect on the labeling of bulk DNA and protein in chromatin fractions.