Über den Nachweis der Carboxyl-Endgruppe im Tabakmosaikvirus durch Spaltung mit Hydrazin

Abstract

It was confirmed by experiments with various peptides and proteins that the procedure described by Akabori for the determination of amino acids with carbonyl end-groups by splitting with hydrazine gives dependable results. Hydrolysis of native TMV and likewise of nucleic acid-free virus protein with hydrazine gave threonin as the only carboxy end-group. The number of end-groups amounted to 2700 per mole TMV which confirmed the results of splitting with carboxypeptidase. Since the number of the amino end-groups is about equally great it is now established that a mole of TMV contains about 2500 peptide chains which agree in their initial and final members.