Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues
Open Access
- 1 March 1973
- journal article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 61 (3), 290-304
- https://doi.org/10.1085/jgp.61.3.290
Abstract
The epithelium of the circumvallate papillae of rat tongues was stripped off by treatment with 0.005% elastase in a state when many taste buds were present. The taste buds were isolated from the stripped epithelium by further treatment with 0.005% elastase and 0.08% trypsin. A protein which was thought to be characteristic of taste buds was found from semimicro disc polyacrylamide gel electrophoretic studies of the stripped epithelia with and without taste buds. This result was supported by micro disc polyacrylamide gel electrophoretic studies of isolated taste buds.Keywords
This publication has 13 references indexed in Scilit:
- Isozymes of α-glucosidase in the proboscis and legs of fliesJournal of Insect Physiology, 1972
- Sweet-sensitive protein from the rat tongue: Its interaction with various sugarsComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1971
- Do Unique Proteins Exist in Taste Buds?The Journal of general physiology, 1971
- On Comparison of Interactions of a “Bitter-sensitive Protein ” from Porcine Tongues with Human Taste ThresholdsNature, 1969
- Putative Bitter-taste Receptor from Porcine TonguesNature, 1969
- Micro-electrophoretic determination of protein and protein synthesis in the 10−9 to 10−7 gram rangeJournal of Chromatography A, 1968
- Sweet-sensitive protein from bovine taste buds. Purification and partial characterizationBiochemistry, 1968
- Free zone electrophoresisChromatographic Reviews, 1967
- The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresisBiochemical Journal, 1967
- Sweet-Sensitive Protein from Bovine Taste Buds: Isolation and AssayScience, 1966