Electrophoretic Study of Tissue Extracts and Sera of Mice After Shock-Producing Injuries

Abstract

The changes which take place in serum and tissue extracts after tourniquet injury have been studied electropho-retically. Upon release of 2-hr. tourniquets, serum albumin rapidly diffuses into the injured tissue, reaching a constant concn. during the 1st hr. The tissue proteins extractable in saline buffered at pH 7.4 decrease steadily during the 16-hr. period studied. No appreciable changes take place in the con-tralateral uninjured leg extracts. The concn. of albumin in the serum decreases after injury, whereas alpha-and beta-globulins increase. The effect of these injuries on the appearance of gamma-globulin has been inconsistent, principally due to the amt. of hemolysis accompanying shock. At pH 7.4, the mobilities of hemoglobin and gamma-globulin are similar. The mobility of the albumin obtained from tissue is significantly greater than that from serum. This change in mobility may be effected by the in vitro contact of tissue with serum.