Abstract
A simple, defined synthetic medium has been developed for the growth of a toxic‐shock‐syndrome‐associated Staphylococcus aureus strain (FRI 1187) in continuous culture, which gives a high growth yield and assayable amounts of acid phosphatase, haemolytic activity, hyaluronate lyase and toxic‐shock‐syndrome toxin‐1. It consists of multiple ions, ammonium sulphate, glucose, cystine, aspartate, glutamate, arginine, glycine, proline, nicotinic acid and thiamine.