Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii
- 1 May 1988
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 2 (3), 315-321
- https://doi.org/10.1111/j.1365-2958.1988.tb00034.x
Abstract
The nucleotide sequence of the nifA gene from Azotobacter vinelandii was determined. This gene encodes an Mr= 58100 polypeptide that shares significant sequence identity when compared to nifA‐encoded products from other organisms. Interspecies comparisons of nifA‐encoded products reveal that they all have a consensus ATP binding site and a consensus DNA binding site in highly conserved regions of the respective polypeptides. The nifA gene immediately precedes the nifB‐nifQ gene region but is unlinked to the major nif gene cluster from A. vinelandii. A potential regulatory gene precedes and is apparently cotranscribed with nifA. Mutant strains that have a deletion or a deletion plus an insertion within nifA are incapable of diazotrophic growth and they fail to accumulate nitrogenase structural gene products.This publication has 34 references indexed in Scilit:
- Identification and Characterization of two nitrogen fixation regulatory regions, nifA and nfrX, in Azotobacter vinelandii and Azotobacter chroococcumMolecular Microbiology, 1988
- The nucleotide sequence of the nifM gene of Klebsiella pneumoniae and identification of a new nif gene: nifZEuropean Journal of Biochemistry, 1987
- Requirements for transcriptional activation in vitro of the nitrogen-regulated gInA and nifLA promoters from Klebsiella pneumoniae: dependence on activator concentrationMolecular Microbiology, 1987
- Sequence of nifL from Klebsiella pneumoniae: mode of action and relationship to two families of regulatory proteinsMolecular Microbiology, 1987
- Nucleotide sequence of the nifLA operon of Klebsiella oxytoca NG13 and characterization of the gene productsMolecular Genetics and Genomics, 1986
- Upstream activator sequences are present in the promoters of nitrogen fixation genesNature, 1986
- Complete nucleotide sequence of the Azotobacter vinelandii nitrogenase structural gene clusterGene, 1985
- Activation of nif gene expression in Azotobacter by the nifA gene product of Klebsiella pneumoniaeNature, 1983
- Nitrogenase. III. Nitrogenaseless mutants of Azotobacter vinelandii: Activities, cross-reactions and epr spectraBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1973
- Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandiiCanadian Journal of Microbiology, 1968