Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Top Cited Papers
- 1 October 2010
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 186 (2), 757-761
- https://doi.org/10.1534/genetics.110.120717
Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.Keywords
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