Effects of cyclophosphamide on murine bone marrow and splenic megakaryocyte‐CFC, granulocyte‐macrophage‐CFC, and peripheral blood cell levels
- 1 August 1982
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 112 (2), 222-228
- https://doi.org/10.1002/jcp.1041120210
Abstract
The effect of cyclophosphamide (CY) on megakaryocytopoiesis in mice was examined with assays of megakaryocyte colony‐forming cells (Meg‐CFC) in bone marrow and spleen and simultaneous determinations of peripheral blood counts, after a single intraperitoneal dose (200 mg/kg) of CY. Significant rebound thrombocytosis (170% of normal) occurred at day 11 after injection with CY, although only modest preceding thrombocytopenia (70% of normal) was observed. After an initial 3–5‐day period of suppression, total megakaryocyte colony‐forming cells (Meg‐CFC) in both bone marrow and spleen of CY‐treated mice demonstrated rebound increases at 5 and 7 days, respectively, after administration of the drug. Granulocyte‐macrophage colony‐forming cells (GM‐CFC) exhibited alterations which were similar to those of Meg‐CFC, suggesting similar sensitivities of Meg‐CFC and GM‐CFC to CY. The increase in Meg‐CFC in both bone marrow and spleen preceded development of thrombocytosis by 4–6 days. This suggests that increased platelet counts in CY‐treated mice are attributable, at least in part, to alterations in feedback mechanisms which control megakaryocytopoiesis, with resultant stimulation of the megakaryocyte progenitor compartment.This publication has 33 references indexed in Scilit:
- The effects of acute thrombocytopenia on megakaryocyte-CFC and granulocyte-macrophage-CFC in mice: studies of bone marrow and spleenBlood, 1980
- Characteristics of murine megakaryocytic colonies in vitroBlood, 1979
- Response of Megakaryocyte, Erythroid, and Granulocyte-Macrophage Progenitor Cells in Mouse Bone Marrow to Gamma-Irradiation and CyclophosphamidePublished by Springer Nature ,1979
- Evidence that Stimulation of Megakaryocytopoiesis by Low Dose Vincristine Results from an Effect on PlateletsBritish Journal of Haematology, 1977
- The effects of colcemid on hematopoiesis in the mouse.JCI Insight, 1976
- Growth of mouse megakaryocyte colonies in vitro.Proceedings of the National Academy of Sciences, 1975
- Effects of Vincristine on Normal and Stimulated Megakaryocytopoiesis in the RatBritish Journal of Haematology, 1975
- A comparison of the effect of cytotoxic agents on agar colony forming cells, spleen colony forming cells, and the erythrocytic repopulating ability of mouse bone marrowJournal of Cellular Physiology, 1973
- VINBLASTINE-INDUCED THROMBOCYTOSISThe Lancet, 1969
- A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASESJournal of Histochemistry & Cytochemistry, 1964