The levels of apolipoprotein-E in hypercholesteroiemic rat serum
- 1 March 1978
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 56 (3), 161-166
- https://doi.org/10.1139/o78-028
Abstract
The levels of apolipoprotein-E (apo-E) in serum and isolated lipoproteins from diet-induced hypercholesterolemic, and to some extent, hypertriglyceridemic rats were measured by electroimmunoassay. The hypocholesterolemia was accompanied by a mild hypertriglyceridemia. The apo-E was increased by 60% in the hypercholesterolemic serum with a 5- and 50-fold increase in very low density lipoproteins (VLDL) and low density lipoproteins (LDL), respectively. However, the proportion of apo-E in nascent VLDL isolated from the hepatic Golgi apparatus of hypercholesterolemic rats was significantly decreased. In control serum, 40-50% of the apo-E is found in the density > 1.21 g/ml fraction, although this is at least partially due to ultracentrifugation. The apoprotein is absent from the density > 1.21 g/ml fraction from hypercholesterolemic serum, suggesting that it is bound more firmly to the lipoprotein complex. The large increases in apo-E in the VLDL and LDL density ranges of serum from hypercholesterolemic rats may in part be accounted for by the utilization of apo-E normally found at higher densities.This publication has 17 references indexed in Scilit:
- Atherogenic hyperlipoproteinemia induced by cholesterol feeding in the Patas monkeyBiochemistry, 1976
- The synthesis of apoproteins of very low density lipoproteins isolated from the Golgi apparatus of rat liverCanadian Journal of Biochemistry, 1976
- Apolipoproteins in rat serum and renal lymph.Proceedings of the National Academy of Sciences, 1976
- Apoproteins of the lipoproteins in a nonrecirculating perfusate of rat liverJournal of Lipid Research, 1976
- Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodiesAnalytical Biochemistry, 1966
- Suggested ModiFications of the Micro Determination of TriglyceridesClinical Chemistry, 1961
- A new color reaction for the quantitation of serum cholesterolClinica Chimica Acta; International Journal of Clinical Chemistry, 1960
- Myocardial Infarction in Rats Fed Diets Containing High Fat, Cholesterol, Thiouracil, and Sodium CholateCirculation, 1959
- THE DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUMJCI Insight, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951