Functional Analysis of MycE and MycF, Two O‐Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics
- 14 May 2009
- journal article
- research article
- Published by Wiley in ChemBioChem
- Vol. 10 (8), 1297-1301
- https://doi.org/10.1002/cbic.200900088
Abstract
Mg motors: We characterized the in vitro function of MycE and MycF, two O‐methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S‐adenosyl‐L‐methionine (SAM)‐dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg2+. With the reconstituted in vitro assays, the order of mycinamicin VI→III→IV in the post‐PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.Keywords
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