Integration of synthetic globin genes into an E. coli plasmid
Open Access
- 1 August 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (8), 1961-1972
- https://doi.org/10.1093/nar/3.8.1961
Abstract
Rabbit globin mRNA has been purified and used as a template by reverse transcriptase. The resulting duplex molecule consisting of rabbit globin mRNA/cDNA has been linked in vitro to Eco RI cleaved plasmid Col E1 DNA. Transformation of E. coli C600 by this recombinant molecule has been achieved. Transformed bacteria acquire the colicin E1 immunity of Col E1 and a closed circular DNA species of4.40–4.45 × 106 daltons in molecular weight, an increase of 2.0 – 2.5 × 105 daltons compared to that of the parent plasmid DNA. In addition, 3HcDNA synthesized from globin RNA hybridizes preferentially to the recombinant plasmid DNA.Keywords
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