Ganglioside patterns and cholera toxin-peroxidase labeling of aggregating cells from the chick optic tectum
- 1 September 1979
- journal article
- research article
- Published by Wiley in Journal of Neurobiology
- Vol. 10 (5), 429-440
- https://doi.org/10.1002/neu.480100502
Abstract
The ganglioside compositions of the chick optic tectum and aggregating tectal cell cultures were examined. Both showed similar trends in changes in ganglioside patterns during development. GD and GD1b were the predominant gangliosides early in development, while GD1a and several other multisialogan gliosides increased in relative amounts with increasing age in vivo and in vitro. Four gangliosides were present early in development which have not previously been reported. These gangliosides are not present at later developmental times suggesting a possible role for them during the critical early stages of nervous tissue differentiation. Some differences were noted when comparing in vivo versus in vitro ganglioside patterns; these differences may possibly be due to the lack of normal retinotectal connections in the cultures. Cytochemical studies on the localization of the presumed cholera toxin‐peroxidase binding site GM1 showed conjugate binding correlates with increasing levels of GM1 in the cultures. In older cultures, the conjugate was uniformly localized on all cells and processes in the aggregates. The conjugate also bound to synaptic membranes and intensely stained the synaptic cleft. This latter observation suggests an enrichment of GM1 in the synaptic cleft region.Keywords
This publication has 29 references indexed in Scilit:
- Ganglioside structures and distribution: Are they localized at the nerve ending?Journal of Supramolecular Structure, 1978
- Neuronal and glial differentiation in reaggregation culturesCell and tissue research, 1977
- Ultrastructural study of plasma membrane GM1 in neuroectodermal cells using cholera-peroxidaseJournal of Neurocytology, 1976
- Cholera Toxin-Peroxidase: Changes in Surface Labeling of Glioblastoma Cells with Increased Time in Tissue CultureScience, 1976
- Developmental changes in gangliosides during myogenesis of a rat myoblast cell line and its drug resistant variantsBiochemical and Biophysical Research Communications, 1976
- The lipid composition of isolated brain cells and axonsJournal of Neuroscience Research, 1975
- Differentiation of Aggregating Brain Cell CulturesPublished by Springer Nature ,1973
- Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoidsBiochemical Journal, 1961
- Quantitive estimation of sialic acidsBiochimica et Biophysica Acta, 1957