Isolation and characterization of the intact gastrin precursor from a gastrinoma
- 5 January 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 210 (2), 185-188
- https://doi.org/10.1016/0014-5793(87)81334-0
Abstract
Antibodies to the extreme C-terminal region of human progastrin have been used to monitor the isolation of high-Mr immunoreactive material in a gastrinoma extract. Microsequence analysis of the product revealed amino acid residues in the first 18 positions corresponding to those predicted from the cDNA sequence for preprogastrin starting at position 22; the sequence and immunochemical data together allow the identification of this material as intact progastrin. Implications for gastrin biosynthesis are discussedKeywords
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