Identification of mixed bacterial DNA contamination in broad-range PCR amplification of 16S rDNA V1 and V3 variable regions by pyrosequencing of cloned amplicons
Open Access
- 1 February 2003
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 219 (1), 87-91
- https://doi.org/10.1016/s0378-1097(02)01190-4
Abstract
Using a sensitive and rapid method combining broad-range PCR amplification of bacterial 16S rDNA fragments and pyrosequencing for detection, identifiKeywords
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