An Extraction Method to Quantitate Serotonin in Human Serum, Amniotic Fluid and Urine Samples by HPLC Using 6-Hydroxy Tryptamine as Internal Standard
- 1 June 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 9 (8), 1747-1758
- https://doi.org/10.1080/01483918608076715
Abstract
A method for extracting serotonin (5-HT) from human serum, amniotic fluid and urine samples is described. A mixture of sample, 6-hydroxy tryptamine (6-HT) as internal standard and sodium carbonate is extracted with ethyl acetate. The ethyl acetate layer is mixed with 200 ul of triethylamine phosphate buffer (pH 3.0) used for mobile phase and centrifuged. An aliquot of the aqueous layer was used for HPLC quantitation with amperometric detection. With this method 30 samples can be assayed in 6 hours.Keywords
This publication has 4 references indexed in Scilit:
- Quantitation of Serotonin in Human Plasma, Serum and Cerebrospinal Fluid Samples by HPLC-EC Using 6-Hydroxytryptamine as an Internal StandardJournal of Liquid Chromatography, 1984
- Simultaneous quantification of dopamine, 5-hydroxytryptaine and four metabolically related compounds by means of reversed-phase high-performance liquid chromatography with electrochemical detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1981
- Efficient extraction and mass spectrometric assay of serotonin in biological fluidsJournal of Mass Spectrometry, 1981
- Increased Whole Blood Serotonin Concentrations in Chronic Schizophrenic PatientsArchives of General Psychiatry, 1981