ROUTINE MICROMETHOD FOR DETERMINATION OF URINARY 17-KETOSTEROIDS
- 1 February 1954
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 15 (2), 193-198
- https://doi.org/10.1530/acta.0.0150193
Abstract
The classical method for determining 17-ketosteroids in urine is applied to 1 ml. of urine. The hydrolysis and extraction is carried out in 25 ml. penicillin-flasks. Special wooden stands and covers allow the simultaneous extraction of 10 specimens. Distilled water blanks as well as 3 levels of standard dehydro-isoandrosterone are run simultaneously to provide internal calibration. The coefficient of variation was [plus or minus] 5% at a level of less than 5[mu]g./ml. urine, [plus or minus] 2% for 10[mu]g. and was negligible above 20[mu]g.Keywords
This publication has 4 references indexed in Scilit:
- RAPID MICRO-MODIFICATION OF THE ZIMMERMANN/CALLOW PROCEDURE FOR THE DETERMINATION OF 17-KETOSTEROIDS IN URINEActa Endocrinologica, 1951
- A RAPID METHOD FOR THE DETERMINATION OF TOTAL URINARY 17-KETOSTEROIDSJournal of Clinical Endocrinology & Metabolism, 1947
- INACTIVATION OF ESTRONE AND DIETHYLSTILBESTROL BY MICROORGANISMS1,2Endocrinology, 1943
- Colorimetric determination of substances containing the grouping —CH2.CO— in urine extracts as an indication of androgen contentBiochemical Journal, 1938