A RAPID METHOD FOR THE DETERMINATION OF TOTAL URINARY 17-KETOSTEROIDS
- 1 December 1947
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 7 (12), 795-800
- https://doi.org/10.1210/jcem-7-12-795
Abstract
A method for the rapld clinical detn. of urinary "17-ketosteroids" in vols. of urine as small as 10 ml. is described. Easily available, inexpensive laboratory equipment is used, and the hydrolysis extraction, and assay can be completed within 4 hrs. Optimum conditions for the hydrolysis and extraction of urine vols. from 10 ml. to 50 are given. Heating at 80[degree] for 10 mins. gives the apparent maximum Zimmerman color producing substances. The results of extraction with several solvents are given. Ethyl ether gives the best results. Recoveries are good. Normal value of 38 young men between ages of 18-30 by this method ranges from 10 to 30 with an avg. of 16.0 mg. excretion in 24 hrs.Keywords
This publication has 4 references indexed in Scilit:
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- NEW COLOR REACTION FOR CERTAIN URINARY 17-KETOSTEROIDS1Endocrinology, 1943
- FRACTIONATION OF NEUTRAL URINARY STEROIDS1Endocrinology, 1941
- BIOCHEMICAL STUDIES ON THE MALE HORMONE AS OBTAINED FROM URINE*†Endocrinology, 1934