Expression of c-mos proto-oncogene transcripts in mouse tissues
Open Access
- 1 June 1985
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 315 (6019), 516-518
- https://doi.org/10.1038/315516a0
Abstract
Valuable information about proto-oncogenes and their physiological function has been obtained by studying their expression in normal cells. However, expression of the c-mos gene, the cellular homologue of the transforming gene of Moloney murine sarcoma virus1,2, has not been detected in normal mouse cells or tissues3–6. The conservation of the c-mos open reading frame7–9 strongly indicates that the gene must function during some portion of the animal life cycle, and other lines of evidence10–15 suggested to us that the c-mos proto-oncogene may be expressed at very low levels in normal tissues. We have used a sensitive S1 nuclease assay to screen RNA preparations from mouse tissues and describe here the detection of c-mos-related transcripts especially in mouse embryos, testes and ovaries. The transcripts found in testis RNA are estimated to be ∼1.7 kilobases (kb) long by Northern analysis. S1 analysis demonstrates that the entire mos open reading frame is present. In contrast, we detect ∼1.4-kb transcripts in ovary RNA and at least two major transcripts, ∼2.3 and ∼1.3 kb, in embryo RNA. The latter transcripts have in common sequences of at least 1 kb, representing most of the c-mos open reading frame. The variation in size of the mos transcript in different tissues suggests a novel regulatory mechanism for the expression of this proto-oncogene.Keywords
This publication has 23 references indexed in Scilit:
- Regulated transcription of c-Ki-ras and c-myc during compensatory growth of rat liver.Molecular and Cellular Biology, 1984
- Complete c-mos(rat) nucleotide sequence: presence of conserved domains In c-mosproteinsNucleic Acids Research, 1984
- Expression of cellular myc and mos genes in undifferentiated B cell lymphomas of Burkitt and non-Burkitt types.Proceedings of the National Academy of Sciences, 1983
- Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like elementNature, 1982
- Differential expression of cellular oncogenes during pre- and postnatal development of the mouseNature, 1982
- Human DNA sequence homologous to the transforming gene (mos) of Moloney murine sarcoma virus.Proceedings of the National Academy of Sciences, 1982
- Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells.Molecular and Cellular Biology, 1982
- Nucleotide sequence of the genome of a murine sarcoma virusCell, 1981
- Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells.Proceedings of the National Academy of Sciences, 1980
- Properties of a Normal Mouse Cell DNA Sequence (sarc) Homologous to the src Sequence of Moloney Sarcoma VirusScience, 1980