Ligand-Induced Maturation of Threonine Deaminase

Abstract

The dimeric intermediate substructures of threonine deaminase, which are obtained by alkaline dialysis of the native tetrameric enzyme, are inactive after reassembly unless they are subsequently exposed to maturation-inducing ligands for which the enzyme possesses stereospecific binding sites. The binding of the ligands at these sites precedes the full maturation of the enzyme.