Similarities and differences among specific tRNA's in mammalian tissues.

Abstract

Specific transfer RNA''s of mammalian origin (from organs and cultured cells) were compared by double-labeling and elution from MAK [methylated albumin kieselguhr] columns for changes that might have occurred during differentiation. No major differences were detected between tissues or species of alanine, leucine, lysine, phenyl-alanine, or threonine tRNA. However, differences were observed in the elution profiles of minor rRNA species for glycine and serine. Major differences were observed in the elution patterns of phenylalanine, serine, and tyrosine rRNA derived from Ehrlich ascites tumor cells when compared to normal mouse tissue.