Site of the previous meiotic division defines cleavage orientation in the mouse embryo
- 23 September 2002
- journal article
- Published by Springer Nature in Nature Cell Biology
- Vol. 4 (10), 811-815
- https://doi.org/10.1038/ncb860
Abstract
The conservation of early cleavage patterns in organisms as diverse as echinoderms and mammals suggests that even in highly regulative embryos such as the mouse, division patterns might be important for development. Indeed, the first cleavage divides the fertilized mouse egg into two cells: one cell that contributes predominantly to the embryonic part of the blastocyst, and one that contributes to the abembryonic part. Here we show, by removing, transplanting or duplicating the animal or vegetal poles of the mouse egg, that a spatial cue at the animal pole orients the plane of this initial division. Embryos with duplicated animal, but not vegetal, poles show abnormalities in chromosome segregation that compromise their development. Our results show that localized factors in the mammalian egg orient the spindle and so define the initial cleavage plane. In increased dosage, however, these factors are detrimental to the correct execution of division.Keywords
This publication has 19 references indexed in Scilit:
- The plane of first cleavage is not related to the distribution of sperm components in the mouse.Human Reproduction, 2002
- Sperm entry position provides a surface marker for the first cleavage plane of the mouse zygoteGenesis, 2002
- Patterning of the embryo: the first spatial decisions in the life of a mouseDevelopment, 2002
- Dynein at the cortexCurrent Opinion in Cell Biology, 2002
- Nuclear migration: Cortical anchors for cytoplasmic dyneinCurrent Biology, 2001
- Role for sperm in spatial patterning of the early mouse embryoNature, 2001
- Animal and vegetal poles of the mouse egg predict the polarity of the embryonic axis, yet are nonessential for developmentDevelopment, 2000
- Centrosome movement in the early divisions of Caenorhabditis elegans: a cortical site determining centrosome position.The Journal of cell biology, 1989
- Nuclear Transplantation in the Mouse Embryo by Microsurgery and Cell FusionScience, 1983
- A reexamination of cleavage patterns in eutherian mammalian eggs: Rotation of blastomere pairs during second cleavage in the rabbitJournal of Experimental Zoology, 1975