The precipitation of 3β-hydroxysteroids by digitonin
- 1 September 1953
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 55 (2), 340-346
- https://doi.org/10.1042/bj0550340
Abstract
The precipitation of 3 beta-hydroxysteroids by digitonin was studied in a roughly quanti-tative manner by the method of Haslewood (Biochem. Jour. 41: xli. 1947). 3beta-hydroxysteroids of the 5 alpha-(A/B-trans) series and of the [DELTA]5-unsaturated series are precipitated by digitonin in much higher dilution than are the corresponding steroids of the 5beta-(A/B-cis) series. This difference in behavior with digitonin can be used in the separation of such steroids. The behavior of some 3beta-acetoxysteroids and other compounds with digitonin was studied.Keywords
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