CYTOGENETIC ANALYSIS OF HUMAN RENAL-CARCINOMA CELL-LINES OF COMMON ORIGIN (NC-65)

Abstract

Cytogenetic analyses were performed on 3 clonal cell lines derived from a human renal cell carcinoma and its lymph node metastasis, 2 long-term tissue culture cell lines (NC 65-Sp and NC 65-R) and a serially transplantable tumor line growing on nude mice and brought into culture at the 5th animal passage (NC 65V). Karyotypes were established using banding techniques. Most of the marker chromosomes could be identified and were derived by deletion, inversion, translocation or isochromosome formation of chromosomes 1, 3, 4, 5, 8, 9 and 17. These markers were different from [human cervical carcinoma] HeLa markers. NC 65-Sp had a near diploid chromosome number, NC 65-R a hypotetraploid number and NC 65-V a bimodal chromosome number. Three chromosome markers were shared by the 3 cell lines; NC 65-R and NC 65-V shared an additional set of 4 markers. Markers specific to each line were observed; they demonstrated the independent derivation of the lines and eliminated laboratory cross-contamination. Common markers between the lines confirmed their common tumoral origin.