Sodium Chloride Reduces Production of Curvacin A, a Bacteriocin Produced by Lactobacillus curvatus Strain LTH 1174, Originating from Fermented Sausage
- 1 April 2004
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 70 (4), 2271-2278
- https://doi.org/10.1128/aem.70.4.2271-2278.2004
Abstract
Lactobacillus curvatus LTH 1174, a strain originating in fermented sausage, produces the antilisterial bacteriocin curvacin A. Its biokinetics of cell growth and bacteriocin production as a function of various concentrations of salt (sodium chloride) were investigated in vitro during laboratory fermentations using modified MRS medium. A model was set up to describe the effects of different NaCl concentrations on microbial behavior. Both cell growth and bacteriocin activity were affected by changes in the salt concentration. Sodium chloride clearly slowed down the growth of L. curvatus LTH 1174, but more importantly, it had a detrimental effect on specific curvacin A production (kB) and hence on overall bacteriocin activity. Even a low salt concentration (2%, wt/vol) decreased bacteriocin production, while growth was unaffected at this concentration. The inhibitory effect of NaCl was mainly due to its role as an aw-lowering agent. Further, it was clear that salt interfered with bacteriocin induction. Additionally, when 6% (wt/vol) sodium chloride was added, the minimum biomass concentration necessary to start the production of curvacin A (XB) was 0.90 g (cell dry mass) per liter. Addition of the cell-free culture supernatant or a protein solution as a source of induction factor resulted in a decrease in XB, an increase in kB, and hence an increase in the maximum attainable bacteriocin activity.Keywords
This publication has 83 references indexed in Scilit:
- Growth of the Bacteriocin-Producing Lactobacillus sakei Strain CTC 494 in MRS Broth Is Strongly Reduced Due to Nutrient Exhaustion: a Nutrient Depletion Model for the Growth of Lactic Acid BacteriaApplied and Environmental Microbiology, 2001
- Incidence and principal sources of Listeria spp. and Listeria monocytogenes contamination in processed meats and a meat processing plantFood Microbiology, 1999
- Behaviour of Listeria spp. in naturally contaminated chorizo (Spanish fermented sausage)International Journal of Food Microbiology, 1999
- Bacteriocinogenic lactic acid bacteria for the biopreservation of meat and meat productsMeat Science, 1998
- US position on Listeria monocytogenes in foodsFood Control, 1996
- Inhibition of Listeria in dry fermented sausages by the bacteriocinogenic Lactobacillus sake CTC494Journal of Applied Bacteriology, 1995
- Influence of growth conditions on the production of lactocin 705, a bacteriocin produced by Lactobacillus casei CRL 705Journal of Applied Bacteriology, 1995
- Behaviour of Listeria monocytogenes in meat and its control by a bacteriocin‐producing strain of Lactobacillus sakeJournal of Applied Bacteriology, 1991
- Modelling the growth response of Staphylococcus xylosus to changes in temperature and glycerol concentration/water activityJournal of Applied Bacteriology, 1989
- Model for combined effect of temperature and salt concentration/water activity on the growth rate of Staphylococcus xylosusJournal of Applied Bacteriology, 1987