Abstract
The growth response of Staphylococcus xylosus strain CM21/3 to changes in temperature and water activity (glycerol concentration) was similar to that observed when water activity was adjusted by added NaCl. At each water activity level the effect of temperature on bacterial growth rate was described well by the square root model. TMIN (the notional minimum temperature for growth) was found to be constant and was similar to the value obtained for the same organism grown in media containing NaCl. Growth rate was proportional to glycerol concentration/water activity allowing the combined effect of this factor and temperature to be modelled by substitution of the constant b in the basic square root model by a term for water activity. The observed minimum water activity for growth at the optimum temperature was close to that predicted by the model.