Assignment of a human genetic locus to chromosome 5 which corrects the heat sensitive lesion associated with reduced leucyl-tRNA synthetase activity in ts025Cl Chinese hamster cells
- 1 September 1980
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 6 (5), 667-687
- https://doi.org/10.1007/bf01538645
Abstract
A heat-sensitive (hs) leucyl-tRNA synthetase (leuRS) deficient CHO mutant, ts025Cl, was fused with human leukocytes and hybrids isolated in HAT medium at the nonpermissive temperature. Nineteen heat-resistant (hr) and 14 hs subclones were isolated from four independent primary hybrids and tested for the expression of 24 human isozymes which have been assigned to 17 human chromosomes. Four hr independent subclones and three hs independent subclones were analyzed for the presence of human chromosomes. A pattern of concordant segregation was noted for the hr phenotype, human hexosaminidase B (EC 3.2.1.30) and human chromosome 5. Based on these results, we have defined the human genetic locus which corrects the heat-sensitive lesion in ts025Cl as hr025Cl and have assigned this locus to human chromosome 5. Two hr hybrids exhibited leuRS activity 2.5 and 4 times the leuRS activity of ts025Cl but a wild-type level of activity was not restored. One hs hybrid had only 73% of the leuRS activity exhibited by ts025Cl while another hs hybrid had 1.8 times the leuRS activity of ts025Cl.Keywords
This publication has 49 references indexed in Scilit:
- Mutations in the structural genes of CHO cell histidyl-, valyl-, and leucyl-tRNA synthetasesSomatic Cell and Molecular Genetics, 1978
- Six complementation classes of conditionally lethal protein synthesis mutants of CHO cells selected by3H-amino acidSomatic Cell and Molecular Genetics, 1978
- Regulation of the Biosynthesis of Aminoacid:tRNA Ligases and of †RNAProgress in Nucleic Acid Research and Molecular Biology, 1978
- CHO cell mutants for arginyl-, asparagyl-, glutaminyl-, histidyl- and methionyl-transfer RNA synthetases: Identification and initial characterizationCell, 1977
- The Status of the Gene Map of the Human ChromosomesScience, 1977
- Temperature-Sensitive Mutations in Animal CellsAdvances in Cancer Research, 1977
- Release by human chromosome 3 of the block at G1 of the cell cycle, in hybrids between tsAF8 hamster and human cells.Proceedings of the National Academy of Sciences, 1976
- Selection by [3H] amino acids of CHO-cell mutants with altered leucyl- and asparagyl-transfer RNA synthetasesSomatic Cell and Molecular Genetics, 1975
- A Mammalian Cell Mutant with a Temperature-Sensitive Leucyl-Transfer RNA SynthetaseProceedings of the National Academy of Sciences, 1973
- Interspecific hybridization of somatic cells.Proceedings of the National Academy of Sciences, 1965