Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel.
- 1 November 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (21), 10262-10266
- https://doi.org/10.1073/pnas.89.21.10262
Abstract
Apical nonselective cation channels with an average single-channel conductance of 34 +/- 2.3 pS were found in M-1 mouse cortical collecting duct cells. Channel activity is increased by depolarization and abolished by cytoplasmic calcium removal. Cytoplasmic application of 0.1 mM cGMP decreases channel open probability by 27%. cDNAs corresponding to approximately 40% of the coding region of the photoreceptor channel were isolated by the polymerase chain reaction from M-1 cells and a rat kidney cDNA library. The rat kidney-derived sequence differs by a single base, and the M-1-cell-derived sequence differs by only two bases, from the photoreceptor sequence. A second clone from M-1 cells differs by 20 out of 426 bases from the photoreceptor sequence. In all three clones, the deduced amino acid sequence is identical to that of the rat photoreceptor channel. Northern blot analysis of poly(A)+ RNA from M-1 cells reveals the presence of a 3.2-kilobase band hybridizing with a retinal cGMP-gated cation channel probe. The results suggest the expression in M-1 cells of more than one gene coding for nonselective cation channels or channel subunits, one of which is identical to the cGMP-gated cation channel gene of rod photoreceptors.Keywords
This publication has 26 references indexed in Scilit:
- Apical membrane ionic channels in the rabbit cortical thick ascending limb in primary cultureBiochimica et Biophysica Acta (BBA) - Biomembranes, 1991
- Similarities in Amino Acid Sequences of Drosophila eag and Cyclic Nucleotide-Gated ChannelsScience, 1991
- cGMP-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitterNeuron, 1991
- Potassium permeable channels in primary cultures of rabbit cortical collecting tubuleKidney International, 1991
- Characterization of a mouse cortical collecting duct cell lineKidney International, 1991
- Developmental and tissue-specific expression of the rod photoreceptor cGMP-gated ion channel geneBiochemical and Biophysical Research Communications, 1990
- Characterization of a 25-pS nonselective cation channel in a cultured secretory epithelial cell lineThe Journal of Membrane Biology, 1990
- Cation Channels in the Apical Membrane of Collecting Duct Prinicipal Cell Epithelium in CultureKidney and Blood Pressure Research, 1990
- Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channelNature, 1989
- Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epitheliumThe Journal of Membrane Biology, 1979