An analytical procedure for the acid-soluble phosphorus compounds in rat-skeletal muscle

Abstract

A method is presented for the quantitative estimation of 95% of the phosphate compounds present in a perchloric acid extract of tissues. The nucleotide components are adsorbed on Norit charcoal and eluted with aqueous pyridine before their separation by paper chromatography. After removal of the inorganic phosphorus and creatine phosphate-phosphorus as the phosphomolybdic acid complex, the glycolytic intermediates are precipitated as barium salts. After removal of barium with cation-exchange resin the solutions are lyophilized and the compounds separated by paper chromatography. Results are presented showing the application of this technique to rat-skeletal muscle.