Technique to Examine Human Sperm Chromosomes Using Zona-Free Hamster Eggs
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Archives of Andrology
- Vol. 12 (1), 79-83
- https://doi.org/10.3109/01485018409161153
Abstract
In experiments with repeated semen samples from two donors, zona-free hamster eggs were used for activation of human spermatozoa to metaphase. When mean number of spermatozoa per penetrated egg was low (1.0—3.3) after insemination in vitro, further incubation resulted in activation of spermatozoa to metaphase. High sperm/egg ratios, 4.2 and 7.5, yielded no sperm chromosomes. The preincubation and insemination intervals necessary for low sperm/egg ratios were individual.Keywords
This publication has 7 references indexed in Scilit:
- DIRECT CHROMOSOMAL ANALYSIS OF HUMAN-SPERMATOZOA - PRELIMINARY-RESULTS FROM 18 NORMAL MEN1982
- Penetration of Human Spermatozoa into the Human Zona Pellucida and the Zona-Free Hamster Egg: A Study of Fertile Donors and Infertile PatientsFertility and Sterility, 1980
- Penetration of the Zona-Free Hamster Egg by Human SpermFertility and Sterility, 1980
- Analysis of Human Spermatozoal Fertilizing Ability Using Zona-Free OvaFertility and Sterility, 1979
- Direct analysis of the chromosome constitution of human spermatozoaNature, 1978
- The Use of Zona-Free Animal Ova as a Test-System for the Assessment of the Fertilizing Capacity of Human SpermatozoaBiology of Reproduction, 1976
- An Air-Drying Method for Chromosome Preparations from Mouse EggsCytogenetic and Genome Research, 1966